I have a 10-kb PCR amplicon whose conc. drops drastically upon DpnI digestion. What could be the reason? How to improve yield?

We have a PCR amplicon which is around 10kb (plasmid backbone). We have to digest it with DpnI before proceeding with other steps. The gel extraction concentration is generally 1ug-1.5ug per 100ul...

24 April 2018 8,282 3 View