Mana hk

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Questions related from Mana hk

What is going wrong with my plasmid DNA transformants? Why I get plasmid DNA transformants smaller than my original pDNA vector?

I am trying to clone my PCR products. I perform double digest (XbaI, NotI) for my plasmid vector (5.3 kb) (Our stock pET28b (+) exist in DH5alpha)and insert gene (1.0 kb). I perform ligation using...

04 December 2016 467 3 View

Why when I use pfu and taq DNA polymerase, I see different size bond in completely same reaction?

My desired gene size is nearly1000 bp. when I use pfu DNA polymerase and taq mastermix the PCR product size is 1000bp but when I use taq DNA polymerase (both Roche and Qiagen) it is 1500 bp.is...

26 November 2016 518 5 View