Man Hon Tsang

3 Questions 3 Answers 0 Followers

Questions related from Man Hon Tsang

What is the reasons of missing the original bands after cutting out DGGE bands and rerun PCR and DGGE?

I have cut out the interested bands out with blades, cut them into piece and wash them with NF water, stored them in NF water in 4 degree overnight, redo PCR and check with agarose gel, then rerun...

04 April 2015 9,189 2 View

What maybe the cause of failure of extracting DNA from DGGE gel bands?

I have excised around 90 bands from two DGGE gels and put them into 2 ml tubes separately, I washed them twice with NF water and then added 50ul NF water to a level that just cover them, I put...

25 March 2015 3,373 3 View

What maybe the cause of failure of extracting DNA from DGGE gel bands?

I have excised around 90 bands from two DGGE gels and put them into 2 ml tubes separately, I washed them twice with NF water and then added 50ul NF water to a level that just cover them, I put...

03 March 2015 777 4 View