Mamuna Mukhtar

7 Questions 12 Answers 0 Followers

Questions related from Mamuna Mukhtar

Can we use any other chemicals than DOPE or DOTAP chloride for the production of cationic liposomes for mRNA encapsulation?

DOPE: Dioleoylphosphatidylethanolamine DOTAP: 1,2-Dioleoyl-3-trimethylammonium-propane Chloride

09 July 2024 1,788 1 View

While performing docking analysis, how do we use the original ligand in receptor protein as a positive control?

While performing docking analysis the protein structure is taken after removing the previously bound ligand from the active site. How do we use the original ligand as a positive control for...

11 April 2023 10,125 4 View

How much quantity of cDNA is required to obtain 50ug IVT mRNA?

As we know that 1 ug/ul of RNA would yeild 1 ug/ul of cDNA. I have to perform in vitro transcription of cDNA into mRNA and how much amount of cDNA will result up to 50ug (0.05mg) of mRNA?

18 November 2022 6,978 4 View

Can I document PyMol free trail version?

I have a 30 days (No License) free trial for PyMol for visualization of my Docking results, can I document it in my research paper?

23 August 2022 8,343 1 View

I am designing an mRNA based vaccine and secondary structure prediction reveals that my protein does not have an alpha helix, how bad is that?

Secondary structure prediction

24 March 2022 9,582 1 View

Hi Everyone! I am designing a Dengue vaccine computationally and need help with how should I perform molecular docking of proteins and epitopes?

I have taken B cell epitopes from consensus protein and overlapping T cell epitopes from B Cell epitopes having MHC class I and II binders. Is is enough if I just dock B cell epitopes with protein...

23 March 2022 4,518 5 View

TrRosetta is a better structure predictor than AlphaFold.

I came across a lot of suggestions to use AlphaFold for 3-D structure prediction of my proteins but I have experienced that I always get better results with trRosetta than AlphaFold and the...

01 January 1970 3,196 5 View