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Hi, I am trying to tag a protein endogenously in Human iPSC model. While reading the protocols, I found different plasmids and I got confused which one works best for hiPCSs. Can anyone help me...
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I was reading the protocol to isolate the stress granules which is a very tedious procedure and required a certain set of reagents to be very specific. I don't understand that why Heparin is used...
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Do I need to perfuse the mouse with PBS before collecting the spleen for the fusion in monoclonal antibody generation?
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Hi, I am generating antibody. I have done it before but for rabbits not for mice. The problem is the antigen injection volume is so less (50ul) that I am unable to make a emulsification. Can...
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I want to do one differentiation assay in pre adipocytes 3T3 L1 cell lines and I have tried the transfection with calcium phosphate method and X-fect clontech reagent, but I m unable to get...
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