Mamta Verma

6 Questions 6 Answers 0 Followers

Questions related from Mamta Verma

What vectors (Plasmids) are required for CRISPR/Cas9 mediated gene knock in?

Hi, I am trying to tag a protein endogenously in Human iPSC model. While reading the protocols, I found different plasmids and I got confused which one works best for hiPCSs. Can anyone help me...

11 May 2021 2,565 3 View

Why Heparin is commonly used in Lysis Buffer?

I was reading the protocol to isolate the stress granules which is a very tedious procedure and required a certain set of reagents to be very specific. I don't understand that why Heparin is used...

23 July 2019 8,562 2 View

Is the perfusion required before the spleen collection?

Do I need to perfuse the mouse with PBS before collecting the spleen for the fusion in monoclonal antibody generation?

04 January 2019 3,077 3 View

Emulsification for Antigen injection in Mice?

Hi, I am generating antibody. I have done it before but for rabbits not for mice. The problem is the antigen injection volume is so less (50ul) that I am unable to make a emulsification. Can...

13 November 2018 1,006 3 View

How can I trypsinise the HepG2 cell line?

I have to check the expression of some gene related to hepatocytes. I have frozen stock of HepG2 cells but after thawing the cells are growing fine with defined morphology but when i do...

12 December 2015 6,874 13 View

Have somebody done the transfection in 3T3 L1 cell lines? How I can get good transfection efficiency in these cells?

I want to do one differentiation assay in pre adipocytes 3T3 L1 cell lines and I have tried the transfection with calcium phosphate method and X-fect clontech reagent, but I m unable to get...

05 September 2015 8,896 5 View