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Questions related from Malwina Radwańska
Dear Researchers, Currently I am struggling with generation of stable mammalian cell line expressing my protein of interest (POI) fused to NanoLuciferase. Unfortunately, when I run Western Blot...
12 December 2018 7,568 1 View
Hello, I'm purifying protein which lacks aromatic residues and contains very little hydrophobic residues. Thus, the only way to measure the protein concentration is BCA assay, as Bradford gives...
01 February 2018 8,188 4 View
Hi, I'm trying to refine the structure of double-mutated protein at 2.4 A resolution. For MR I used the structure (not fully refined, 2.75A Res) of the Wild Type. After first round of refinement I...
20 March 2017 2,436 6 View
Hi, I'm purifying Fab proteins but I have no time to finish it today so that I would like to continue the next day (I know i shouldn't but have no choice). Right now I'm centrifuging cell lysate...
27 September 2016 4,358 4 View
I'm trying to crystallize complex of Fab with antigen. I used various screen, and the only crystals I obtained (completely reproducible) are spherulites in JSCG+ commercial screen. I obtained them...
14 January 2016 697 7 View
Can anyone help me - I mutated protein, (point Ala mutation) so the products should be the same molecular weight. One of them is mutated correctly (1st mutant) but the other one didn't gave the...
26 November 2015 3,980 3 View
It especially concerns 9th well. Will diluting my SB sample with water help?
20 May 2015 6,562 30 View
