Malwina Radwańska

4 Questions 3 Answers 0 Followers

Questions related from Malwina Radwańska

Why expression of NanoLuciferase-POI fusion protein in mammalian cells yields expression of both fusion protein and free NanoLuc?

Dear Researchers, Currently I am struggling with generation of stable mammalian cell line expressing my protein of interest (POI) fused to NanoLuciferase. Unfortunately, when I run Western Blot...

12 December 2018 7,530 1 View

Can I flash freeze cleared E. coli cell lysate and store in -80 for further purification?

Hi, I'm purifying Fab proteins but I have no time to finish it today so that I would like to continue the next day (I know i shouldn't but have no choice). Right now I'm centrifuging cell lysate...

27 September 2016 4,318 4 View

How can I optimize spherulite crystals?

I'm trying to crystallize complex of Fab with antigen. I used various screen, and the only crystals I obtained (completely reproducible) are spherulites in JSCG+ commercial screen. I obtained them...

14 January 2016 663 7 View

Can anyone help me, why my SDS-PAGE gel smears like that?

It especially concerns 9th well. Will diluting my SB sample with water help?

20 May 2015 6,514 30 View