Maliheh Parvank

2 Questions 3 Answers 0 Followers

Questions related from Maliheh Parvank

How can I trunsduce iPSCs with lentivrus vector?

Hi, I wanna trunsduce iPSCs with lentivirus for differentiation. There are some problems. I grow them on MEF, and I think this feeder will become trunsduced more than my colons. And another...

16 April 2017 9,613 2 View

Can someone provide tips on altering IPSc to single cells, and then culturing them??

I need a protocol for doing this! and also I wanna know if the single cells of breaking IPSc should be treated with IPSc complete media(F12, bfgf, KOSR) or usual media(DMEM high glu, FBS)?

21 February 2017 6,549 5 View