Maliheh Davari

3 Questions 2 Answers 0 Followers

Questions related from Maliheh Davari

Could Lipofectamine alone affect gene expression?

Hello everyone I am transfecting my primary endothelial cells using Lipofectamine LTX. As controls, besides other controls such as non-transfected cells and the transfected cells with empty...

02 May 2025 8,556 1 View

Could ApoI enzyme cut restriction site of EcoRI?

Hello for cloning my gene of interest into a plasmid, i placed BglII and ApoI sites into the ends of my gene but there is a EcoRI site in my gene sequence. i want to know whether ApoI will cut...

07 August 2018 2,299 5 View

Does anyone have any experience in measuring Transepithelial Electrical Resistance of epithelial cells cultured on Transwell Filters?

Hello i want to measure TEER of retinal pigment epithelial cells cultured on transwell filters but the voltohmmeter being used in our lab show variation in a wide range around 1000 ohm. wanna to...

24 February 2018 5,619 1 View