Malgorzata Majewska

5 Questions 7 Answers 0 Followers

Questions related from Malgorzata Majewska

Which kit would you recommend for isolation of RNA from dicotyledonous plants (leaves, stems and roots)? Which vendor would you recommend generally?

My plant material is rich in secondary metabolites. With the isolated RNA I would like to examine expression of interested genes in Real Time PCR.

11 November 2019 3,339 8 View

Why I see extra bands after single restriction enzyme digestion (EcoRI) but not after double digestion (EcoRI + HindIII)?

After double digestion of my plasmid DNA with EcoRI and HindIII I have obtained two bands as expected. But when I digested the plamid DNA only with EcoRI to linearize it, I obtained three bands...

12 December 2018 4,164 4 View

Could you recommend some reliable company that offer a cloning service?

question as above

10 October 2018 925 6 View

What upstream sequence of translation initiation site (AUG) should I use to obtain high protein expression in dicot Salvia milthiorrhiza?

question as above

10 October 2018 7,211 5 View

Which Kozak sequence should I use to obtain high protein expression in plants, especially in dicot Salvia milthiorrhiza?

question as above

10 October 2018 4,388 2 View