How to efficiently transfer 1.5mm SDS gel on PVDF?

Hello, I have issues with transferring 1.5mm thick SDS-PAGE gels (14% acrylamide, with saccharose) on PVDF membrane. I tried 50 min @ 100V in Towbin buffer with 10% Met-OH, but most of the...

03 July 2019 2,032 4 View