Malcolm Lim

4 Questions 10 Answers 0 Followers

Questions related from Malcolm Lim

Clathrin-dependent endocytosis inhibitor?

I want to inhibit clathrin-mediated endocytosis of antibody-targeted receptor using chlorpromazine-HCl as inhibitor. The hypothesis is that chlorpromazine will inhibit clathrin-dependent...

12 December 2017 7,412 1 View

Pressure cooker Antigen retrieval, Same Heating profiles for ALL buffers?

I use the pressure cooker for antigen retrieval. Citrate buffer:125 deg for 5m then 90deg for 10m, EDTA buffer: 105 deg for 15m then 90deg for 10m. Just wonder if the heating profile can be same...

10 October 2016 7,029 2 View

How do I optimize IHC antibody?

Gene discovery usually leads to knock down studies etc and one will usually end up requiring to test and optimise the antibody for the protein in question. Currently, I have western blots to test...

12 December 2015 5,561 3 View

How can I use immunofluorescence to detect the same target located at both membrane and nucleus in cellcultured coverslip?

I am trying to detect phosphokinase receptor in my cells using the same antibody via IF. The antigen may be located at the membrane or nucleus. Do I detect the membrane first followed by a second...

11 November 2015 6,515 8 View