Malaika Gomes

5 Questions 2 Answers 0 Followers

Questions related from Malaika Gomes

DAB (3'3 diaminobenzidine) will not dissolve?

Hi, i ordered DAB from sigma: https://www.sigmaaldrich.com/catalog/product/sial/d8001?lang=en®ion=CA and I tried dissolving it in water, MES, and even pH lowered to 3 it still wont...

01 January 2020 2,299 4 View

What do you use to clear Arabidopsis embryos in NBT/DAB staining ??

For NBT staining of Arabidopsis embryos, I notice that they get darker after I add ethanol (70%), but not if I add chloral hydrate ? I also notice that they don't usually get darker for 100%...

10 October 2019 4,531 1 View

Why does my 4-MUG assay show GUS expression for Columbia?

I am doing a 4-MUG assay for GUS expression in seeds. I imbibe them for 6 hours and 9 hours (two separate experiments). Is this a basal level of fluorescence? I used 1 mM 4-MUG. I also measured...

04 April 2019 9,362 5 View

Golden Gate always giving an empty vector?

I am trying to synthesize CRISPR constructs, using Golden Gate to put my sgRNAs in the pHEE401E vector. Whenever I do colony PCR with my original primers, it appears that the reaction worked as I...

01 January 2019 7,904 4 View

For CRISPR, does it matter if the guide RNA is looking for the antisense or sense strand?

So, I've used the CRISPR P 2.0 software to look up guide RNAs to use to delete a region in my gene's promoter. Past members of our lab were using one guide RNA taken straight from that website to...

11 November 2018 7,766 2 View