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Questions related from Malaika Gomes
Hi, i ordered DAB from sigma: https://www.sigmaaldrich.com/catalog/product/sial/d8001?lang=en®ion=CA and I tried dissolving it in water, MES, and even pH lowered to 3 it still wont...
01 January 2020 2,296 4 View
For NBT staining of Arabidopsis embryos, I notice that they get darker after I add ethanol (70%), but not if I add chloral hydrate ? I also notice that they don't usually get darker for 100%...
10 October 2019 4,529 1 View
I am doing a 4-MUG assay for GUS expression in seeds. I imbibe them for 6 hours and 9 hours (two separate experiments). Is this a basal level of fluorescence? I used 1 mM 4-MUG. I also measured...
04 April 2019 9,359 5 View
I am trying to synthesize CRISPR constructs, using Golden Gate to put my sgRNAs in the pHEE401E vector. Whenever I do colony PCR with my original primers, it appears that the reaction worked as I...
01 January 2019 7,903 4 View
So, I've used the CRISPR P 2.0 software to look up guide RNAs to use to delete a region in my gene's promoter. Past members of our lab were using one guide RNA taken straight from that website to...
11 November 2018 7,763 2 View