Maksim Kazarnovskiy

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Questions related from Maksim Kazarnovskiy

Can primary CD4 T cells be expanded without beads?

Hello, world! Have anyone ever expanded human or murine CD4+ T cells without CD3/CD28 beads? Is it possible? We are triing to do it using RPMI-1640 with glutamine, ME, Il-2 on the plates covered...

21 October 2016 2,904 3 View

Are Amaxa Nucleofector kits somehow interchangeble (neet to nucleofect human T cells with hMSC kit)? Can buffers be prepared in the lab?

Hello, everyone! Today I got Amaxa Nucleofector II for my work. I haven`t read all the manual yet, but as far as I could find out, there are different kits for almost all cell types. I got a...

20 September 2016 9,512 4 View