Majid Pahlevan Kakhki

3 Questions 8 Answers 0 Followers

Questions related from Majid Pahlevan Kakhki

The luciferase signal is too low but the renilla is OK in dual luciferase assay? What's the problem?

Hello I'm trying to do the luciferase assay with my manipulated vector but I'm getting very low luciferase signal. The positive controls for luciferase and renilla are OK and i'm getting a high...

07 July 2018 636 4 View

Does anyone have experience in the PBMCs culture?

Please share your protocol here and let me see the image of cultured cells.

06 June 2016 8,780 1 View

Does anybody have experience with isolation of monocytes (without any antibody) from the primary culture of PBMCs?

In my first experience, I cultured the PBMCs and isolate the adherent cells to the flask after 10 hours. After about 2 days the morphology of cells changed from a round small shaped-cells to the...

03 March 2016 8,166 4 View