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Hello! I´m developing a sandwich ELISA. My assay recognizes protein denaturalized very well, but there isn´t recognition when it is in its native form. So maybe if I heat the sample I could detect...
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Hello. I have a problem. I am expressing a protein in the SoluBL21 strain at two temperatures (18°C and 20°C). At 18°C the pellet was beige while at 20°C it was gray. Generally, in other cultures...
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