Maidoly Batista Fernández

3 Questions 5 Answers 0 Followers

Questions related from Maidoly Batista Fernández

What volume would you recommend adding to a well of a 12-well plate?

Hi everyone, I am conducting an experiment to induce p-PI3K activation using EGF. However, I am unsure about the appropriate volume of my medium + EGF mixture to add to each well of a 12-well...

20 August 2025 5,341 2 View

What is the optim temperature for protein denaturation in supernatants??

Hello! I´m developing a sandwich ELISA. My assay recognizes protein denaturalized very well, but there isn´t recognition when it is in its native form. So maybe if I heat the sample I could detect...

19 April 2024 5,743 3 View

What could have happened to have obtained different colored bacterial pellets?

Hello. I have a problem. I am expressing a protein in the SoluBL21 strain at two temperatures (18°C and 20°C). At 18°C the pellet was beige while at 20°C it was gray. Generally, in other cultures...

01 January 1970 5,379 5 View