Maibam Singh

4 Questions 12 Answers 0 Followers

Questions related from Maibam Singh

Not able to form seal as I used to. Did/changed everything that could contribute to it but still can't form giga seal. Could it be the slice health?

I am doing whole cell electrophysiology on mice midbrain slice (coronal) from 5-9 weeks old mice. To be specific, I am recording from GABAergic cells from substantia Nigra reticulata. In the past...

09 September 2019 1,517 9 View

Is it possible to loose nascent fluorescence (EYFP) in slices due to longer storage?

Hi, I use a EYFP cre reporter mice and do IHC on them to see the expression pattern. Usually, I slice the brain and on the same day start the IHC protocol with blocking and primary antibody...

10 October 2018 7,507 1 View

Anyone facing the problem with whole cell patching due to increasing osmolarity of the neuronal culture cell media?

I am doing whole cell patch clamp electrophysiology on acute cultures of midbrain dopaminergic cells. I normally start doing my experiments on DIV 7 and go as far as DIV 12. However, I have...

08 August 2017 7,185 6 View

Why do i get contamination with primary culture of dopaminergic neurons from mouse brain?

I have had consistent contamination in my primary culture of mouse dopaminergic neurons and I am not able to culture the cells beyond 5 days. From visual inspection, I believe its a fungal...

08 August 2015 5,792 7 View