Mahmoud Hefny Gad

4 Questions 19 Answers 0 Followers

Questions related from Mahmoud Hefny Gad

Could anyone help me with alpha amylase assay by DNS?

Currently I am working with alpha amylase assay using DNS method but I have a problem with the colour which is not changing even I used high concentration from the phenolic standards (Trolox and...

08 August 2015 3,813 13 View

Can anyone help to find the best protocol for evaluation of alpha glucosidase inhibition assay of plant extracts?

Dear all, I tried to evaluate an alpha glucosidase assay of the methanol plant extract using this protocol: 50µL of sample (100-1000 µg/ml) + 100 µL of α-glucosidase (0.2 u/ml) + 50 µL of p-NPG...

07 July 2015 7,307 9 View

Could any one help me to do oxygen radical absorbance capacity (ORAC) assay for my sample?

I am working with a herbal plants and i wish to do ORAC assay for my samples to evaluate the antioxidant activity. Indeed, my problem is i don't know what is the suitable concentration should i...

01 January 2015 1,170 3 View

How can I do cytotoxicity assay in vitro? And is there any fast methods to know the activity of my fractions?

I am working on separation and purification of the active compounds from the secondary metabolites of medicinal plants by different techniques of chromatography, but I want to know if can I do any...

03 March 2014 1,605 17 View