Mahmoud Farghali

3 Questions 10 Answers 0 Followers

Questions related from Mahmoud Farghali

How much Lipofectamine RNAimax shoiuld be used for siRNA transfection?

Hi all, I am trying to transfect HepG2 cells with siRNAs using Lipofectamine RNAimax. I did the transfection when the cells were 80%-85% confluent. I used 12 well-plate with 50nM siRNAs final...

08 August 2018 7,023 12 View

Looking for a protocol for isolation of nuclei from mammalian cells?

I am trying to get pure cytoplasmic and nuclear fractions from mammalian cells after infection, for further viral DNA quantification. After fractionation and DNA extraction, I quantified...

10 October 2017 3,408 4 View

Hello, I need to generate stable cell lines expressing number of nucleoporins fused to GFP, Does anyone have experience on how to generate them?

Each cell line would express one of the nucleoporins fused to GFP.

02 February 2016 7,899 3 View