Mahmoud Fassad

3 Questions 5 Answers 0 Followers

Questions related from Mahmoud Fassad

Sanger sequencing interpretation?

I have found homozygous mutation in a certain gene using NGS (c.436G>C) and when I tried sanger confirmation and segregation within the family. I got these two chromatograms; one for the...

13 October 2016 7,395 4 View

Is there any way for haplotype calling from Bam files?

I wonder if anyone could tell me if it is possible to define the haplotype associated with certain mutation using Bam files and if this is possible which software I can use and the reliability if...

30 August 2016 5,935 1 View

Protocol for RNA extraction from Transwell inserts?

I wonder if someone has a direct protocol to extract RNA from 6.5 mm Transwell inserts in which the cell growth area is 0.33 cm2?

26 August 2016 3,550 3 View