Mahesh S Dhar

2 Questions 2 Answers 0 Followers

Questions related from Mahesh S Dhar

Protein uptake by mammalian cells - what methodologies can be employed to make the protein cross the plasma membrane onto the cytoplasm?

I want to use an enzyme (21kDa) as therapeutics. I want to know what methodologies can be employed to make the protein cross the plasma membrane onto the cytoplasm. Also, can tagging the protein...

08 August 2014 2,032 4 View

Can anyone help with protein purification?

I have purified a protein of 21 kDa by Ni-NTA. The purified protein shows a single band on SDS-PAGE but three bands on Native-PAGE. The protein is a monomer, as confirmed by FPLC. The protein also...

04 April 2014 5,229 3 View