Mahesh Prakash Bhatta

6 Questions 14 Answers 0 Followers

Questions related from Mahesh Prakash Bhatta

No Survival of NK-92 Cells After Puromycin Selection in Lentiviral Transduction — What Might Be the Reason, and How Can I Optimize?

I have infected NK-92 cells with lentivirus at MOI 30 using protamine sulfate and spinoculation (2,000 rpm for 1 hour, ~600 × g). The following day, I replaced the medium, and after 48 hours, I...

12 August 2025 2,506 2 View

What are those Artifacts in ORO staining and how to resolve this problem ?

I have seen crystal-like artifacts in ORO staining. those artifacts are also ORO positive. does anybody face this problem and has any solution please share. I filtered the stain with a 0.22um...

24 December 2022 6,140 3 View

How to maintain cells confluency in Umbilical cord mesenchymal stem cell culture ?

I am trying to maintain the cell confluency around 90-100% in the glass culture plate. but cells are changing their morphology into spheroid-like structures by shrinking cells and are likely to be...

22 November 2022 9,813 6 View

A patient (under medication) have cholestrol level of 210 mg/dl , after 8 days its 133 mg/dl and again 14 days late,r it goes up to 270 mg/dl.Justify?

Cholesterol, Dyslipidemia

18 October 2017 3,631 7 View

In which condition TSH level is normal but high or low level of FT3 and FT4 found?

Thyroid disorder

03 August 2017 2,120 6 View

Is this possible to have high Anti-TPO level while FT3 ,FT4 and TSH level are in range ?

Biochemistry, Immunology, Thyroid disorder

20 August 2016 2,163 12 View