Mahdi Ghanbari

6 Questions 9 Answers 0 Followers

Questions related from Mahdi Ghanbari

Can someone advise on PCR-Based screening for a gene?

In some articles I have seen that for screening of a specific gene the authors just used a primer set and then sequenced the fragment. But, in other articles, after PCR, fragments were cloned and...

30 May 2014 4,565 3 View

Can anyone please tell me the most reliable media to isolate actinomycetes from a freshwater environment?

13 November 2013 6,676 2 View

Appropriate method for Total DNA Extraction of Intestinal Microorganisms in fish?

Any ideas on the effective and economical method for total DNA extraction from intestinal contents in fish?

22 October 2013 9,655 7 View

Suitable way to preserve intestine samples?

What is the appropriate way to preserve fish intestine samples for one or two months for bacteriological studies ? Do I need to add Glycerol to the samples and keep them at -20 degrees C?

12 September 2013 2,628 9 View

MRS or NAP for studying the LAB in smoked salmon?

What is the best medium to isolate lactic acid bacteria from cold smoked salmon? MRS agar, nitrite actidione or polymyxin agar (NAP) with pH 6.2 ?

07 September 2013 6,473 1 View

Low (if any) presence of L.monocytogenes 4b in food

Listeria monocytogenes serotype 4b accounts for most cases of human listeriosis and at the same time it is seldom found in foods, less than five percent in environment. What is the reason? Any idea?

07 July 2013 4,749 2 View