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Questions related from Mahboubi Mohaddese
we cloned a gene in pet28a and got a recominant colony. The insert was 720 bp. we' ve done colony PCR on colony with forward primer of gene and reverse primer of pet. I got a product about 500 bp...
03 January 2017 7,051 8 View
Dear I prepared my cloning reactions with positive and negative controls of my reaction. The positive control was pET with double digestion in presence of T4 DNA ligase and the negative Control...
05 December 2016 7,386 3 View