Mahboubeh Mostafaei

5 Questions 12 Answers 0 Followers

Questions related from Mahboubeh Mostafaei

What is the efficient approach for identifying candidate genes and their functions in cannabis GWAS?

What is the most efficient way to identify candidate genes and their functions for GWAS results in cannabis when the current reference genome only shows LOC IDs and incomplete functional...

14 March 2023 629 3 View

How to convert PED and MAP files (about 600K) to Text file?

I have large PED and MAP files (about 600K) that I am trying to work with them and open them in Excel format. I opened them with EmEditor software and saved as TEXT file and then opened it in...

08 October 2019 7,348 1 View

Do you think High Resolution Melt is a good technology for polymorphism identification?

I decided to amplify my desired gene sequence for some strains of my sample and after that study polymorphism but my gene sequence have a high identity to another gene so I can, t design primers...

15 December 2014 1,198 4 View

What kind of protocol do you suggest for RNA extraction to have an appropriate 260/230 ratio (1.8 - 2.2)?

I need to extract RNA from cannabis. My buffer extraction is Biozol but when I use nanodrop to identify the concentration and quality of my samples, 260/230 ratio was so low. I think my samples...

17 September 2014 2,107 3 View

Can anyone help me solve a problem I've been having with amplification of a specific gene by PCR?

I have a problem with amplification of a specific gene. After gene specific primer designing, I amplify my desired gene with PCR but don't get any answer. My enzyme is DreamTaq DNA polymerase. I...

12 September 2014 7,306 9 View