Mahboubeh Mostafaei

4 Questions 10 Answers 0 Followers

Questions related from Mahboubeh Mostafaei

How to convert PED and MAP files (about 600K) to Text file?

I have large PED and MAP files (about 600K) that I am trying to work with them and open them in Excel format. I opened them with EmEditor software and saved as TEXT file and then opened it in...

08 October 2019 7,326 1 View

Do you think High Resolution Melt is a good technology for polymorphism identification?

I decided to amplify my desired gene sequence for some strains of my sample and after that study polymorphism but my gene sequence have a high identity to another gene so I can, t design primers...

15 December 2014 1,176 4 View

What kind of protocol do you suggest for RNA extraction to have an appropriate 260/230 ratio (1.8 - 2.2)?

I need to extract RNA from cannabis. My buffer extraction is Biozol but when I use nanodrop to identify the concentration and quality of my samples, 260/230 ratio was so low. I think my samples...

17 September 2014 2,086 3 View

Can anyone help me solve a problem I've been having with amplification of a specific gene by PCR?

I have a problem with amplification of a specific gene. After gene specific primer designing, I amplify my desired gene with PCR but don't get any answer. My enzyme is DreamTaq DNA polymerase. I...

12 September 2014 7,286 9 View