Does anybody have experience with high or varying RFU (relative fluorescence unit) values between replicates in qPCR?

I have run a qPCR-assay using a Cy5-probe. On one of the plates, some technical replicates have very high RFU-levels, but the Ct-values of the replicates are almost identical. Does anybody know...

06 March 2020 6,679 4 View