Maghesree Chakraborty

2 Questions 6 Answers 0 Followers

Questions related from Maghesree Chakraborty

Can analytic C18 column be used to purify peptides in lieu of preparative/ semi-preparative columns? Is the protocol different from analytic HPLC?

Column Details particle size :3um, pore size: 120 A, Dim. : 4.6 mmx100 mm

06 June 2016 7,547 8 View

Can someone provide a detailed protocol for sample and solvent preparation for HPLC analysis of peptides?

I am new to peptide synthesis and purification. My peptides are in aqueous solution containing 5% TFA. I intend to use UHPLC from Thermo scientific for analysis.

10 October 2015 9,504 4 View