Madhvi Kanchan

5 Questions 3 Answers 0 Followers

Questions related from Madhvi Kanchan

Sequencing error or unexpected deletion?

I introduced a point mutation in my protein and cloned the mutated gene into the pCold vector. After sequencing, I observed a gap of 2 base pairs upstream of the mutation site. Considering the gap...

20 June 2025 432 3 View

What is the best temperature and incubation time for successful ligation of blunt ends?

I have performed it at 16 degree for overnight and at 21degree for 2 hours but not getting appropriate results.

26 August 2022 4,596 4 View

Not getting band on gel after elution of digested product. Why?

Elution of two digestions A (1.5+5kb) and B (10kb) was performed at same time with same kit, getting band of good concentration for A (1.5 the required one) but not getting any results for B. We...

26 August 2022 3,117 1 View

My agrobacterium (LBA4404 pCambia **) stabs are not growing on both LB and LA?

I did streak plate from glycerol stock. Cultures were grown on plate and in LB, they grew well initially. But when I reinoculated glycerol stock after 3 months on LB and LA media their is no sign...

25 August 2021 7,054 4 View

How long can a double digested plasmid be stored after cleanup/purification? Is it okay if i store the double digested plasmid in -20deg after cleanup

Last month I stored a double-digested vector backbone after cleanup/purification at -20 degrees and did ligation with my insert but got a self-ligated product. Is it possible that its overhangs...

01 January 1970 3,197 4 View