Madhuram Khandelwal

4 Questions 9 Answers 0 Followers

Questions related from Madhuram Khandelwal

Problem in trypsinization of cell line

I am working on Bladder cancer cell line that is HT 1376. I am having problem in trypsinization. For trypsinization after removing media from flask, first we wash with PBS, after that we add 2 ml...

21 January 2016 9,251 11 View

No result in overlap PCR , any suggestions?

I amplified two DNA fragment 314bp and 854 bp length by using two primers sets AB and CD. Reverse primer of first set that is B and Forward primer of second set that is C they are complementary to...

02 September 2015 475 8 View

Does anyone having experience working with Decitabine?

I am working on bladder cancer cells. I am using Decitabine drug for my study. For experiments i dissolved drug in DMSO and after that i aliquoted it and stored it in -80. Every time i used a...

17 October 2014 4,400 4 View

What is the best method for RNA isolation from tissue samples?

I am working on human tissue samples. I am studying expression of some genes. For that I have to make cDNA from RNA which I isolate from tissue samples. We can use various methods like chopping,...

24 December 2013 1,757 12 View