Madhura Ramani

4 Questions 3 Answers 0 Followers

Questions related from Madhura Ramani

Im doing mycoplasma detection by doing PCR and i need help with Primers ?

Hi everyone, i am doing mycoplasma detection by doing Pcr and im doing PCR 10ul reaction, my primer concentration os 2pmol and vol is 0.1ul. As i dont have pipette for 0.1ul i am thinking of...

02 February 2020 8,167 3 View

Can i grow hacat and L929 cells with dmem high glucose + 4mM glutamine?

I am gonna grow hacat and L929 cells and can i grow these cell line with dmem high glucose+ 4mM L glutamine? Which is more preferable 2mM or 4mM glutamine? Thanks in advance.

03 March 2019 549 3 View

How can i confirm the activity of peptide from the amino acid sequence?

is there any software or tool which would tell us the activity of peptide from the amino acid sequence??

03 March 2019 2,443 2 View

Can I use DMEM high glucose + 4M glutamine in culturing the L929 cell line??

I am going to grow L929 cells for scratch assay and i have been told that i need to grow them on DMEM low glucose with 2M glutamine and 10% fbs . can i grow it on high glucose with 4M glutamine...

02 February 2019 8,092 2 View