I am using pET28a plasmid, when i run a gel of pET28a plasmid i got 3 band. when i doing double digestion, digestion will not takes place.why?

Restriction enzyme that i used for double digestion is EcoR1 and Xho1 for 1,2 and 3 hours but didnt get digested band on gel. RE is of Genei company.

02 May 2024 3,581 1 View