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Questions related from Madeline Lewis
I am trying to identify submucosal immune cells in samples of rat gut tissue via IHC, but need a good marker/antibody for these cells. Does anyone have any suggestions as to which marker may be...
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I am trying to isolate neutrophils from rat whole blood, aprox. 5 mL collected via cardiac puncture into syringes containing 1mL ACD. I am pipetting whole blood directly onto 5 mL of Histopaque...
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I'm trying to use ACK lysis buffer to lyse RBCs from whole blood samples after an initial lysing with H2O (restoring isotonicity with 10% NaCl). My last step is using RIPA buffer + protease...
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I am trying to figure out the best protocol for lysing neutrophils after a gradient isolation procedure from whole blood. If anyone could pass along a protocol that has worked well I would greatly...
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I am trying to isolate neutrophils from bovine and rat whole blood samples. I have purchased the Dynabeads Epoxy and antibody linking kit and have a bovine CD44 and rat NCF2 antibody that can be...
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