Madeleine Sutherland

3 Questions 2 Answers 0 Followers

Questions related from Madeleine Sutherland

When to add protease inhibitors to C43(DE3) cell lysate?

I'm expressing a his6-MBP-tagged membrane protein under the araBAD promoter in C43 cells, because I've shown that with the same construct growing in C43 pushes more of the protein into the soluble...

15 November 2019 2,718 3 View

What AraBADC- strains do you recommend for membrane protein expression?

I am going to boldly attempt the purification of a viral membrane protein and want to try several expression systems to see what works best. One I would like to try is pBAD because (in my hands...

19 June 2019 4,047 1 View

What is the average number of compounds included in a QSAR study? Has anyone done statistics on this?

I'm asking because it seems to vary widely. Some authors claim to have done "SAR" with 4-10 compounds, which seems silly because the "relationship" between descriptors/structural features and...

26 July 2018 6,178 3 View