M Siva Sai Naga Anurag

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Questions related from M Siva Sai Naga Anurag

Should we be preparing fresh 1X Annexin V binding buffer every time?

Hello everyone, Should we be preparing 1X concentration of Annexin V binding buffer just before the experiment or Is it okay if we use previously made cold 1X binding buffer? I've two...

11 November 2019 10,009 4 View

No bands observed in Western blot analysis of Notch 4 proteins obtained from normal and carcinoma cell lines. Could anyone help me out??

I've done Western blot analysis of Notch 4 proteins obtained from normal and carcinoma cell lines. Transfer is complete onto NC membrane. I've prepared new antibodies with dilutions 1:750. I tried...

05 May 2016 6,268 4 View