Maheshwaran Velusamy

3 Questions 5 Answers 0 Followers

Questions related from Maheshwaran Velusamy

Why am I getting aggregation in gold nano particle synthesis ?

We have synthesized gold nanoparticles by trisodium citrate reduction method. After two days we got fibre like aggregation in our samples. Please refer the image. Is it contamination or...

04 April 2019 9,291 4 View

How can we ensure that we adequately lysed the overexpressed protein pellet by sonication?

Protein was expressed in SHuffle cells in presence of 0.5%glycerol in the LB media(230ml). pellet was dissolved in 20ml lysis buffer(50mM Tris pH 8.0, 150mM Nacl, 10% glycerol). i couldnt see...

01 January 2019 9,252 7 View

I would like to know the stability of DTT in Tris buffer with respect to pH and temperature?

Am trying to purify my protein in Tris-Hcl pH 8.0 with DTT. I am keeping the protein for overnight dialysis. I found that, around this pH 8.5 in phosphate buffer stability of DTT is about 1.4...

12 December 2018 386 12 View