M. Arefian

2 Questions 3 Answers 0 Followers

Questions related from M. Arefian

Can someone help with my RT-PCR to reduce unspecific amplification?

There is more than ONE band during 2-steps reverse transcription PCR with a gene specific primer in my gel (Lanes 3 and 4) at higher molecular weights. I used revers primer in synthesize of cDNA....

13 August 2014 6,387 16 View

Does anyone have experience with RT-PCR?

Which one is better in synthetic of first strand cDNA during reverse transcription: Revers Primer or Forward primer or both? I believe the Revers one is useful. Is it quite true? With many Thanks..

12 August 2014 5,807 13 View