Does someone have experience with Angiotensin II Enzyme Immunoassay (EIA) Kit for angiotensin II in serum samples?

I had used Angiotensin II EIA Kit cat.no. RAB0010 from SIGMA but the results are confusing... I cannot understand why standart curve must be built by 1,000 pg/ml, 100 pg/ml, 10 pg/ml, 1 pg/ml, 0.1...

30 January 2015 7,855 4 View