Lyna M'Rabet

3 Questions 9 Answers 0 Followers

Questions related from Lyna M'Rabet

How to get rid of Bacillus subtilis spores?

I work with the cell-free supernatant of a Bacillus subtilis bacterial culture. After centrifugation, I use 0.2 micrometer filters to collect the supernatant. I noticed that spores pass through...

19 October 2022 9,769 5 View

Proteinase K seems to partially digest my product, any explanation?

Testing for proteic nature of a putative bacteriocin: I used 1mg/ml Proteinase K to digest the putative bacteriocine in a cell free supernatent (with unknown concentration, but the supernatant...

11 January 2022 4,741 10 View

pH issue with supernatant of a Lactobacillus?

I am currently working on Lactobacillus salivarius for an eventual bacteriocin production, I don't adjust the pH of the supernatant when realizing the deep well diffusion agar test. To check...

15 July 2021 1,335 5 View