Why do my bacteria die after phage infection?

When I was amplifying the phage library, TG1 cells with an OD600 of 0.4-0.6 were infected with M13KO7 at an MOI of 20 and incubated at 37°C for 1 hour. Subsequently, I centrifuged the cells and...

21 April 2025 6,069 2 View