Lulong Bo

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Questions related from Lulong Bo

Is anyone familiar with Cre/loxP for gene conditioning knockout mice?

We are creating a gene conditioning knockout mice strain by Cre/loxP. I think the first step is to examine the physlogical difference between it with WT. What parameters should I examine? such as...

02 February 2015 5,998 5 View

Looking for illustratation graph for macrophage phagocytosis ?

Although I can find some good dirgram from google images search, I want to make a new one including the molecure I am interested. Does anybody here know what kind of software can I choose? OR Does...

01 January 2015 3,918 0 View

Can I use RAW264.7 to replace Kupffer cells for in vitro study?

I want to do some experiments on Kupffer cells. I have done it in vivo. However, it's a little difficult to isolate and culture Kupffer cells for me. My question is can I use RAW264.7 to repalce...

01 January 2015 1,067 4 View

Cold or warm buffer for alveolar macrophage isolation from BAL? what is your choice?

I am trying to isolate the AM from BAL of mice. I read several articles and protocols. Some of them use cold buffer. Some use prewarmed buffer (37℃). Although the content of the buffer migh be the...

01 January 2015 1,296 3 View

How can I define the resolution phase of acute lung injury?

There are increasing research on the resolution phase of acute lung injury. However, what is the standard of the resolution phase? I see the term in lots of literature. There is different time...

01 January 2015 9,396 4 View