Luiz Alexandre V Magno

2 Questions 6 Answers 0 Followers

Questions related from Luiz Alexandre V Magno

Does anyone know if SP6 RNA polymerase works on DNA templates under a T7 promoter?

I only have this enzyme in the lab to promote in vitro transcription. I know I should get another DNA template subcloned with a SP6 promoter, however I am very curious to know if SP6 RNA...

04 April 2014 8,520 4 View

How could we evaluate (in vitro) if phosphate modifications at specific sites in proteins affect drug binding?

At some point in drug development of kinase inhibitors, we should know if the drug can bind (at similar affinities) to the both phosphorylated and un-phosphorylated forms of kinase proteins .

23 October 2012 3,430 7 View