Luís Sousa

3 Questions 2 Answers 0 Followers

Questions related from Luís Sousa

How to normalize fluorescence measurements from different microplate runs?

I'm currently doing an experiment where I'm following the expression of a reporter gene (GFP) along growth using S. cerevisiae. The cultivations last around 48 hours. I observed that the results...

11 June 2024 5,911 3 View

Why final biomass is not the same from fermentation to fermentation?

Dear all, So I'm running some experiments where I will need to record growth profile data, and collect samples for glucose, ethanol and volatiles analysis. My strategy is always to use use fresh...

08 March 2023 7,745 1 View

Why do I have this discrepancy in GFP vs lacZ reporter genes?

Hey there, I'm now doing an experiment where I want to follow the expression of a gene during a batch culture of yeast (cerevisiae). I first started to fuse my gene promoter of interest with...

09 October 2022 2,822 0 View