How can we evaluate the affinity of an antibody towards a specific antigen if the antibody has been expressed on the membrane of E. coli?

Hello! We used plasmid pNeae2 to display nanobodies on the surface of E. coli DH10B-T1 R using the bacterial display methodology. Then we attempted to use this bacterium as a primary antibody in...

05 June 2023 9,972 1 View