Luigi Petrone

3 Questions 6 Answers 0 Followers

Questions related from Luigi Petrone

Should I apply ATR correction while fitting Amide I band in its components?

Usually ATR correction is required for quantitative analysis, but no one ever mentions this for the Amide I band fitting, maybe the components are too close so it is not significant, but still.

28 February 2014 4,814 9 View

How can I fit Amide I band in an IR spectrum?

I need to calculate secondary structure of peptides from an IR spectrum. I wonder how to set baseline of the Amide I band before fitting (original shape is not symmetric being higher at lower...

28 February 2014 8,475 4 View

Does a protein secondary structure changes from dry powder state to water solution?

I am interested especially in the IR spectrum of dry and aqueous type I Collagen. Collagen has triple helical structure in water (between pH 3-12). Does it retain its triple helix in the dry...

19 April 2013 4,176 2 View