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Questions related from Luděk Záveský
I have been wondering what minimal amount of RNA may be successfully used for TaqMan Array MicroRNA cards as the manufacturer recommends 350 – 1000 ng to be used without pre-amplification,...
10 October 2015 5,737 7 View
This software should possibly enable efficiency corrections, normalization procedures and determination of potential endogenous controls based on expression data from TaqMan Array Human MicroRNA...
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Which microRNAs have been identified as good candidates as the stable endogenous controls for small-scale qRT-PCR expression studies of non-cellular urine. Thank you for suggestions.
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RIN number is not applicable in cell-free samples and their RNA. Is it possible to use higher proportion of microRNA in small RNA (analyzed by Small RNA kit, Agilent) as an indicator of better...
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I am looking for a software or algorithm which makes it possible to correct data from real-time PCR microRNAs expression analysis for efficiency and interplate calibration (single assays used)....
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