Why does my Phospho-4E-BP1 (Thr37/46) Antibody bind between 100-150kDa and not 15-20kDa?

Hi everyone. So I have tried to optimize my phospho-antibodies for western Blotting, however after several attempts the one antibody, Phospho-4E-BP1, keeps binding to the wrong protein. The...

20 October 2020 3,963 2 View