How can I detach/elute exosomes (Vesicles) from CD-63 coated beads?

I´m trying to perform a 2D electrophoresis with exosomes isolated with CD63 positive beads. We first performed a 1D with silver staining. We lysed exosomes bound to beads with SDS buffer. Silver...

14 May 2014 6,179 7 View