Lucía Yim

2 Questions 8 Answers 0 Followers

Questions related from Lucía Yim

Bacterial total RNA extraction (including small RNAs) using mirVana renders only molecules under 200nt???

I am trying to extract total RNA from bacteria (Salmonella) using the mirVana kit. I am interested in studying the total trasncriptome, inlcuding small RNAs. But after extraction (I elute in...

12 December 2017 389 7 View

Protein bound to Glutathione agarose even after thrombin digestion

I am trying to purify a protein as fusion to GST, but after glutathione agarosa column binding and in column thrombin digestion I can't elute my protein from the colum (even if in an SDS-PAGE gel...

08 August 2012 6,722 19 View