Lucia Brandimarte

5 Questions 6 Answers 0 Followers

Questions related from Lucia Brandimarte

How can I reduce luminescence signal which seems too high?

I’ m performing dual luciferase assay experiments using 1 µg of pgl4.10[luc2] and 100ng of pgl4.74[RLuc] as reporter control ( firefly: renilla 10: 1). My transfection efficiency on HeLa cells...

05 May 2015 2,271 6 View

How can I reduce random mutations during a cloning experiment?

Hello! I'm trying to insert a 2400 bp long sequence in pGEM-Teasy vector. Although the long insert was difficult to clone I got a few colonies but after sequencing they showed some random...

01 January 2015 4,864 7 View

Blue white selection problems, any suggestions?

I'm cloning a PCR product in pGEMTeasy on DH10b electroMAX cells (Invitrogen). The problem is that after transformating my colonies were light blue and not dark blue so I couldn't discriminate...

11 November 2014 7,731 6 View

Can anyone help with dual luciferase assay on HL60 cell lines?

I'm trying to evaluate the effect of a mutation in the promoter region on translation using dual luciferase assay. I transfected HL60 cell line using 4 ug of pGL4.10 [luc2] and 444ng of pGL4.74...

08 August 2014 6,645 2 View

What is the best method to transfect an OCI cell line?

Is Nucleofector technology the best method to transfect OCI cell line for luciferase assay experiment or is FuGENE reagent also ok?

02 February 2014 5,082 1 View