Lucia Taja

2 Questions 10 Answers 0 Followers

Questions related from Lucia Taja

Which is the best transfection method for cell lines that growth in suspension?

We are trying to transfect lymphoma cell lines, and we have tried several methods, the best is through the use of lentivirus of course, but in this pandemic days is very difficult to import this...

07 July 2020 8,205 12 View

Could any one give some advise on amplifying DNA extracted from paraffin embedded tissue?

I have added DTT to the extraction buffer, and or I have boiled DNA prior the PCR reaction, I have also used kits and no-kits for the extraction but the PCR results are still poor.

24 November 2015 8,625 6 View