Luca Murru

6 Questions 18 Answers 0 Followers

Questions related from Luca Murru

Why i'm not able to induce a stable DHPG mediated LTD in my preparation?

Hi everyone, I'm trying to induce chemical LTD at the CA3-CA1 hippocampal synapse using DHPG 100 uM as reported in literature but at the moment i'm not able to induce a stable LTD after 10 minutes...

05 May 2016 6,053 14 View

How can i record from dopaminergic neurons of the substantia nigra pars compacta?

Hi collegues,I'm going to perform some recordings on dopaminergic neurons of the substantia nigra pars compacta. Do you have some advices on the cutting procedure? I tried coronal slicing but i...

03 March 2016 1,431 6 View

How can i analyse sodium currents from I-V relationship?

Hi people, I performed some experiments in Ca1 pyramidal neurons to study sodium and potassium currents. I used classic I/V recordings (10 mV steps starting from -80mV with the cell clamped to...

02 February 2016 1,214 7 View

What do you think about animal facility, stress and LTP induction?

Hi all, I'm in troubles with some LTP experiments in those days. I'm trying to perform some experiments for a paper revision but i cannot induce LTP. Obviously before submitting the paper i've...

12 December 2015 2,185 14 View

Which is the best blade inclination to cut very healthy coronal hippocampal slices?

Hi scientists, i have some questions for you. I'm trying to performe fEPSP experiments to induce LTP in hippocampal CA1, but i have some problems finding a good response. I would to record fEPSPs...

12 December 2015 7,404 3 View

Can anyone help me with problem with a membrane test pClamp 10?

Hi everybody, I have some problems. Today I decided to clean my setup disconnecting and reconnecting some cables to ameliorates the noise. I disconnected the digidata from the PC removing the USB...

07 July 2014 5,191 12 View